DNA Sequencing Core Facility
The DNA Sequencing Core Facility provides DNA sequencing services to investigators at CMMT and CFRI, as well as off site researchers. It is equipped with the ABI Prism 3130xl Genetic Analyzer, a 16-capillary fluorescence-based DNA analysis system.
In addition to DNA sequencing, the 3130xl system supports a variety of applications including comparative sequencing, DNA fragment analysis, and genotyping. Skilled staff members are ready to lend their expertise in sequencing PCR products, plasmids, and BACs with a turn-around time of 1 to 3 days.
Since 2002, the facility has run over 96,000 analyses of samples for over 165 research groups at the CMMT, CFRI, UBC, SFU, UVic, and numerous hospital, industry and government research groups.
Sequencing is performed on an ABI 3130xl 16-capillary automated genetic analyzer. Samples are typically run on a 50 cm capillary yielding 800 to 900 bp with results typically available in one to three business days. We offer both Full Service sequencing of your DNA templates, or a Ready-to-Run service if you prefer to do your own sequencing reactions.
We sequence single or double stranded DNA from plasmids, PCR products, BAC, PAC and cosmid templates as well as sequencing of bisulfite converted methylated DNA utilizing the ABI BigDye v3.1 Terminator chemistry. Alternate chemistries (ABI BigDye V1.1 and dRhodamines) are utilized as required for difficult to sequence templates.
Sequence data is provided electronically as analyzed ABI files (.ab1), though are available in other formats (see protocol notes).
As successful sequencing reactions are highly dependent on the quality and accurate quantitation of templates provided, please adhere to the following points for all samples submitted:
A more economical DNA sequencing option is available for plasmids and PCR products. The Pre-Mixed Samples Service special pricing is available if the following sample submissions requirements are followed:
Please provide the facility with your cycle sequenced and purified sequencing products (in 8-strip PCR tubes or 96-well plates if more than 6 samples). The facility will run your samples, confirm sequencing results, and provide results in ABI .ab1, or your preferred electronic format. See our protocol notes for details.
DNA sequencing pricing for Academic researchers, effective August 1st, 2016. Private sector pricing is available upon request.
The following DNA sequencing pricing applies to CMMT and CFRI Academic researchers, effective August 1st, 2016.
Additional information on fees:
Invoicing will be processed on a monthly basis by Journal Voucher for UBC customers and by invoice for non-UBC customers. Payment should be made upon receipt of invoice. HST is applicable to all invoices unless paid by UBC Journal Voucher.
DNA fragment analysis is performed on the ABI Prism 3130xl Analyzer. Our facility can analyze fragments from 50-1000bp in size from AFLP, and microsatellite markers (see protocol notes). Size standards are provided by the facility. Please contact us for further details and for pricing for your project.
Click here to download the sequencing request form that can either be printed or sent as an email attachment. If your browser displays the Excel file, then you can print directly from your browser, or choose 'File > Save As' to save the file to your disk.
Samples can dropped off at the CFRI shipping and receiving at 950 West 28th Avenue (before 4:00 pm), by calling extension 3814 on the Oak Street entrance intercom, or by courier to the address below. On site researchers may drop off samples in the facility's freezer in room 3028.
For your convenience, we have set up a service contact form. Please feel free to use this form to contact us regarding our laboratory services.
Ms. Liz Hui
+1 (604) 875-3814
CMMT/CFRI DNA Sequencing Core Facility
Attention: Liz Hui
The Centre for Molecular Medicine and Therapeutics
950 West 28th Avenue, Room A3-193
V5Z 4H4 Canada
Direct: +1 (604) 875-3814
Fax: +1 (604) 875-2494